Part:BBa_K2260000:Design

PhaCBA operon w/ Histidine tags

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The phaCBA operon was codon optimized for use in E. coli using this codon optimization table. Four restrictions sites present in the original operon obtained from BBa_K1149051 were removed, allowing our part to be compatible in all iGEM RFC standards. New restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the N-termini of each protein in the operon for protein purification.

Source

The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.